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ProductsBack/AHR antibody [RPT1]
IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100

AHR antibody [RPT1]

GTX22770
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Primate, Rat
TargetAHR
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Overview

  • Supplier
    GeneTex
  • Product Name
    AHR antibody [RPT1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:100-1:1000. IHC-P: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RPT1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID196
  • Target name
    AHR
  • Target description
    aryl hydrocarbon receptor
  • Target synonyms
    FVH3, RP85, bHLHe76, aryl hydrocarbon receptor, AH-receptor, ah receptor, aromatic hydrocarbon receptor, class E basic helix-loop-helix protein 76
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35869
  • Protein Name
    Aryl hydrocarbon receptor
  • Scientific Description
    The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human, Mouse, Porcine, Primate, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndrome.
    Read this paper
  • Mono-(2-ethyl-5-hydroxyhexyl) phthalate promotes uterine leiomyoma cell survival through tryptophan-kynurenine-AHR pathway activation.
    Read this paper
  • NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma. Zhan Y et al., 2022 Oct 19, BMC Med
    Read this paper
  • TDO2 and tryptophan metabolites promote kynurenine/AhR signals to facilitate glioma progression and immunosuppression.
    Read this paper
  • The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis. Ma X et al., 2022, Front Microbiol
    Read this paper
  • Exposing human primary dermal fibroblasts to particulate matter induces changes associated with skin aging. Reynolds WJ et al., 2020 Nov, FASEB J
    Read this paper
  • IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression. Sadik A et al., 2020 Sep 3, Cell
    Read this paper
  • Aryl hydrocarbon receptor-ligand axis mediates pulmonary fibroblast migration and differentiation through increased arachidonic acid metabolism. Su HH et al., 2016 Aug 31, Toxicology
    Read this paper
  • Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues. Mariuzzi L et al., 2016 Sep, Lab Invest
    Read this paper

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Figure 1. Western blot analysis of AHR using anti-AHR antibody (A00225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # A00225-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 100 kDa. The expected band size for AHR is at 96 kDa.
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