IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![WB analysis of 20 ug of HepG2, U2OS, and K562 whole cell lysates using GTX22770 AHR antibody [RPT1]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22770/GTX22770_1587_WB_w_23060620_783.webp "WB analysis of 20 ug of HepG2, U2OS, and K562 whole cell lysates using GTX22770 AHR antibody [RPT1]. Dilution : 1:1000")
![ICC/IF analysis of NIH-3T3 cells using GTX22770 AHR antibody [RPT1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22770/GTX22770_414_ICC-IF_w_23060620_585.webp "ICC/IF analysis of NIH-3T3 cells using GTX22770 AHR antibody [RPT1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
AHR antibody [RPT1]
GTX22770
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Primate, Rat
TargetAHR
Overview
- SupplierGeneTex
- Product NameAHR antibody [RPT1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:100-1:1000. IHC-P: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRPT1
- ConjugateUnconjugated
- Gene ID196
- Target nameAHR
- Target descriptionaryl hydrocarbon receptor
- Target synonymsah receptor; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; bHLHe76; class E basic helix-loop-helix protein 76; RP85
- HostMouse
- IsotypeIgG1
- Protein IDP35869
- Protein NameAryl hydrocarbon receptor
- Scientific DescriptionThe protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]
- ReactivityHuman, Mouse, Porcine, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndrome.Read more
- Mono-(2-ethyl-5-hydroxyhexyl) phthalate promotes uterine leiomyoma cell survival through tryptophan-kynurenine-AHR pathway activation.Read more
- NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma. Zhan Y et al., 2022 Oct 19, BMC MedRead more
- TDO2 and tryptophan metabolites promote kynurenine/AhR signals to facilitate glioma progression and immunosuppression.Read more
- The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis. Ma X et al., 2022, Front MicrobiolRead more
- Exposing human primary dermal fibroblasts to particulate matter induces changes associated with skin aging. Reynolds WJ et al., 2020 Nov, FASEB JRead more
- IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression. Sadik A et al., 2020 Sep 3, CellRead more
- Aryl hydrocarbon receptor-ligand axis mediates pulmonary fibroblast migration and differentiation through increased arachidonic acid metabolism. Su HH et al., 2016 Aug 31, ToxicologyRead more
- Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues. Mariuzzi L et al., 2016 Sep, Lab InvestRead more
- Severe liver cirrhosis markedly reduces AhR-mediated induction of cytochrome P450 in rats by decreasing the transcription of target genes. Floreani M et al., 2013, PLoS OneRead more