
Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 (GTX27900) 7.5% SDS PAGE GTX113306 diluted at 1:1000
AIF antibody [N1C1]
GTX113306
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAIFM1
Overview
- SupplierGeneTex
- Product NameAIF antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9131
- Target nameAIFM1
- Target descriptionapoptosis inducing factor mitochondria associated 1
- Target synonymsAIF, AUNX1, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, SEMDHL, apoptosis-inducing factor 1, mitochondrial, apoptosis-inducing factor, mitochondrion-associated, 1, auditory neuropathy, X-linked recessive 1, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor, testicular secretory protein Li 4
- HostRabbit
- IsotypeIgG
- Protein IDO95831
- Protein NameApoptosis-inducing factor 1, mitochondrial
- Scientific DescriptionThis gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161



![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113306/GTX113306_40128_20160804_WB_shRNA_watermark_w_23060501_176.webp)
![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000. Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113306/GTX113306_40128_20160107_WB_R_liver_w_23060501_213.webp)






![AIF antibody [N1N2], N-term detects AIFM1 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: AIFM1 protein stained by AIF antibody [N1N2], N-term (GTX102399) diluted at 1:500. Blue: Hoechst 33343 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102399/GTX102399_39925_IFA_w_23060100_355.webp)