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Western Blot analysis of HuvEc cells using AIM2 Polyclonal Antibody
Western Blot analysis of HuvEc cells using AIM2 Polyclonal Antibody
Western Blot analysis of HuvEc cells using AIM2 Polyclonal Antibody

AIM2 Antibody

CSB-PA000838
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAIM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AIM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9447
  • Target name
    AIM2
  • Target description
    absent in melanoma 2
  • Target synonyms
    interferon-inducible protein AIM2; PYHIN4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14862
  • Protein Name
    Interferon-inducible protein AIM2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AIM2 using anti-AIM2 antibody (PB9683). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIM2 antigen affinity purified polyclonal antibody (Catalog # PB9683) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIM2 at approximately 45 kDa. The expected band size for AIM2 is at 39,40-45 kDa.
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