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Western blot All lanes: Adenylate kinase 2, mitochondrial antibody at 5ug/ml Lane 1: Hela whole cell lysate Lane 2: Mouse kidney tissue Lane 3: Mouse small intestine tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 26, 23, 15, 25, 22 kDa Observed band size: 27 kDa
Western blot All lanes: Adenylate kinase 2, mitochondrial antibody at 5ug/ml Lane 1: Hela whole cell lysate Lane 2: Mouse kidney tissue Lane 3: Mouse small intestine tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 26, 23, 15, 25, 22 kDa Observed band size: 27 kDa
Western blot All lanes: Adenylate kinase 2, mitochondrial antibody at 5ug/ml Lane 1: Hela whole cell lysate Lane 2: Mouse kidney tissue Lane 3: Mouse small intestine tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 27, 26, 23, 15, 25, 22 kDa Observed band size: 27 kDa

AK2 Antibody

CSB-PA001509ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetAK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID204
  • Target name
    AK2
  • Target description
    adenylate kinase 2
  • Target synonyms
    ADK2, adenylate kinase 2, mitochondrial, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, adenylate kinase isoenzyme 2, mitochondrial, adenylate monophosphate kinase, testis secretory sperm-binding protein Li 220n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54819
  • Protein Name
    Adenylate kinase 2, mitochondrial
  • Scientific Description
    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AK2 using anti-AK2 antibody (PB10034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AK2 antigen affinity purified polyclonal antibody (Catalog # PB10034) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AK2 at approximately 26 kDa. The expected band size for AK2 is at 26 kDa.
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