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Western Blot analysis of Human liver cancer tissue using AKR1D1 Polyclonal Antibody at dilution of 1:650
Western Blot analysis of Human liver cancer tissue using AKR1D1 Polyclonal Antibody at dilution of 1:650
Western Blot analysis of Human liver cancer tissue using AKR1D1 Polyclonal Antibody at dilution of 1:650

AKR1D1 Polyclonal Antibody

E-AB-10761
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AKR1D1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.2mg/ml
  • Scientific Description
    The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKR1D1 using anti-AKR1D1 antibody (A05278-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1D1 antigen affinity purified polyclonal antibody (Catalog # A05278-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1D1 at approximately 37 kDa. The expected band size for AKR1D1 is at 37 kDa.
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