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Western Blot analysis of Human liver cancer tissue using AKR1D1 Polyclonal Antibody at dilution of 1:450
Western Blot analysis of Human liver cancer tissue using AKR1D1 Polyclonal Antibody at dilution of 1:450
Western Blot analysis of Human liver cancer tissue using AKR1D1 Polyclonal Antibody at dilution of 1:450

AKR1D1 Polyclonal Antibody

E-AB-14530
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetAKR1D1
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Overview

  • Supplier
    Elabscience
  • Product Name
    AKR1D1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6718
  • Target name
    AKR1D1
  • Target description
    aldo-keto reductase family 1 member D1
  • Target synonyms
    3o5bred, CBAS2, SRD5B1, aldo-keto reductase family 1 member D1, delta(4)-3-ketosteroid 5-beta-reductase, delta(4)-3-oxosteroid 5-beta-reductase, steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51857
  • Protein Name
    Aldo-keto reductase family 1 member D1
  • Scientific Description
    The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of AKR1D1 using anti-AKR1D1 antibody (A05278-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1D1 antigen affinity purified polyclonal antibody (Catalog # A05278-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1D1 at approximately 37 kDa. The expected band size for AKR1D1 is at 37 kDa.
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