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AKR7A2 / AFAR Antibody

LS-C331369
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetAKR7A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AKR7A2 / AFAR Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 39kDa, while the observed MW by Western blot was 35kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:10 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 39kDa, while the observed MW by Western blot was 35kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8574
  • Target name
    AKR7A2
  • Target description
    aldo-keto reductase family 7 member A2
  • Target synonyms
    AFAR, AFAR1, AFB1-AR1, AKR7, aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, AFB1-AR 1, HEL-S-166mP, SSA reductase, aflatoxin aldehyde reductase, aflatoxin beta1 aldehyde reductase, aldoketoreductase 7, epididymis secretory sperm binding protein Li 166mP, succinic semialdehyde reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKR7A2 using anti-AKR7A2 antibody (A06949-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR7A2 antigen affinity purified polyclonal antibody (Catalog # A06949-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR7A2 at approximately 36 kDa. The expected band size for AKR7A2 is at 35-40 kDa.
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