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Akt antibody

20R-1920
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    Akt antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613) 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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