More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Akt (Ser-473), Phosphospecific Antibody

Akt (Ser-473), Phosphospecific Antibody

BSM-70330M
Bioss
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetAKT1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    Akt (Ser-473), Phosphospecific Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    WB(1:300-5000), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M114
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Storage Instruction
    -20°C,-80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
References
GeneTex
AKT1 antibodyGTX110613
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetAKT1
  • SizePrice
Western Blot analysis of A549 cells using Akt Polyclonal Antibody
Product group Antibodies
Cusabio
AKT1/AKT2/AKT3 AntibodyCSB-PA000848
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-AKT1EB06875
ApplicationsWestern Blot, ELISA
TargetAKT1
  • SizePrice
Product group Antibodies
MedChem Express
AKT1 Antibody (YA834, Mouse)HY-P80007
TargetAKT1
  • SizePrice
Product group Antibodies
Biosynth
Akt antibody20R-1920
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-pAKT [AbAb-pAKT]AB04102-10.0
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetAKT1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-AKT1 Antibody, Affinity PurifiedA302-065A
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetAKT1
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-AKT1 AntibodyA285951
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-AKT1 Antibody144-11016
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-AKT1,2,3 Antibody Picoband(r)A00024-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetAKT1
  • SizePrice