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ProductsBack/AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.16C8.25F6]
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates (p/n W10-000-358). Lane 2: PDGF stimulated NIH/3T3 cell lysates (p/n W10-001-377). Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500) in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates (p/n W10-000-358). Lane 2: PDGF stimulated NIH/3T3 cell lysates (p/n W10-001-377). Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500) in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates (p/n W10-000-358). Lane 2: PDGF stimulated NIH/3T3 cell lysates (p/n W10-001-377). Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500) in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.

AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.16C8.25F6]

TA396766
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetAKT1
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1 mg
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Overview

  • Supplier
    OriGene
  • Product Name
    AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.16C8.25F6]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14E5.16C8.25F6
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    AKT Antibody
  • Reactivity
    Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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Akt1 antibody immunoprecipitates Akt1 protein in IP experiments. IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells. A. 30 μg whole cell extract of Akt1-protein expressing 293T cell B. Control with 3 μg of preimmune Rabbit IgG C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613) 10 % SDS-PAGE The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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