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Formaldehyde-fixed, paraffin-embedded rat ovary stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded rat ovary stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.
Formaldehyde-fixed, paraffin-embedded rat ovary stained with AKT1-pS473 Rabbit Polyclonal Antibody AE00207 at 10ug/ml for 30 minutes at RT. Epitope retrieval: Microwave heating at pH7 to boiling, 5min at 95-98C, 1min at 100C, then cool to roomtemp. DAB staining by HRP polymer.

AKT1-pS473 Polyclonal Antibody

Research Use Only
AE00207
Aeonian Biotech
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetAKT1
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    AKT1-pS473 Polyclonal Antibody
  • Delivery Days Customer
    3
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Category Supplier
    cancer, nucleus, phospho-specific, rabbit
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Modification Type
    Phosphorylated
  • Scientific Description
    Phospho-specific rabbit polyclonal antibody to human AKT1, phosphorylated S473 (AKT1-pS473)
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity
    Human
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    Avoid repeated freeze/thaw cycles. For long term storage, keep small aliquots at -20°C or -80°C and keep one aliquot at 4°C for daily experimentations. Azide will preserve antibody at 4°C for 6-12 months, when kept away from direct sun light.
  • UNSPSC
    12352203

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Western Blot of immunoprecipitation (lanes 2 and 3) from 200ug Jurkat (10 min stimulation by 100nM Calyculin A) extract (lane 1), using 3ug AKT1-pS473 Rabbit Polyclonal Antibody AE00208, and using the same antibody for staining the blot at 1ug/ml (1h at ambient temp). ECL staining by HRP.
Product group Antibodies
Aeonian Biotech
AKT1-pS473 Polyclonal AntibodyAE00208
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetAKT1
  • SizePrice