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ProductsBack/AKT1-pS473 Polyclonal Antibody AE00208
Western Blot of immunoprecipitation (lanes 2 and 3) from 200ug Jurkat (10 min stimulation by 100nM Calyculin A) extract (lane 1), using 3ug AKT1-pS473 Rabbit Polyclonal Antibody AE00208, and using the same antibody for staining the blot at 1ug/ml (1h at ambient temp). ECL staining by HRP.
Western Blot of immunoprecipitation (lanes 2 and 3) from 200ug Jurkat (10 min stimulation by 100nM Calyculin A) extract (lane 1), using 3ug AKT1-pS473 Rabbit Polyclonal Antibody AE00208, and using the same antibody for staining the blot at 1ug/ml (1h at ambient temp). ECL staining by HRP.
Western Blot of immunoprecipitation (lanes 2 and 3) from 200ug Jurkat (10 min stimulation by 100nM Calyculin A) extract (lane 1), using 3ug AKT1-pS473 Rabbit Polyclonal Antibody AE00208, and using the same antibody for staining the blot at 1ug/ml (1h at ambient temp). ECL staining by HRP.

AKT1-pS473 Polyclonal Antibody AE00208

AE00208
Aeonian Biotech
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetAKT1
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    AKT1-pS473 Polyclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31749
  • Protein Name
    RAC-alpha serine/threonine-protein kinase
  • Scientific Description
    AKT1-pS473 Polyclonal Antibody AE00208
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.
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