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Alanine Aminotransferase (ALT) Polyclonal Antibody

CAU28354
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetGpt
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Overview

  • Supplier
    Biomatik
  • Product Name
    Alanine Aminotransferase (ALT) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.47 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID76282
  • Target name
    Gpt
  • Target description
    glutamic pyruvic transaminase, soluble
  • Target synonyms
    1300007J06Rik, 2310022B03Rik, ALT, ALT1, Gpt-1, Gpt1, alanine aminotransferase 1, GPT 1, glutamate pyruvate transaminase 1, glutamic pyruvic transaminase 1, soluble, glutamic--alanine transaminase 1, glutamic--pyruvic transaminase 1
  • Host
    Rabbit
  • Protein IDQ8QZR5
  • Protein Name
    Alanine aminotransferase 1
  • Scientific Description
    The Alanine Aminotransferase (ALT) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alanine Transaminase/Gpt using anti-Alanine Transaminase/Gpt antibody (A01638-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alanine Transaminase/Gpt antigen affinity purified polyclonal antibody (Catalog # A01638-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alanine Transaminase/Gpt at approximately 55 kDa. The expected band size for Alanine Transaminase/Gpt is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-Alanine Transaminase/Gpt Antibody Picoband(r)A01638-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetGpt
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