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ALAS1 Antibody

LS-C748510
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetALAS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ALAS1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 12kDa/70kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 12kDa/70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID211
  • Target name
    ALAS1
  • Target description
    5'-aminolevulinate synthase 1
  • Target synonyms
    ALAS, ALAS-H, ALAS3, ALASH, MIG4, 5-aminolevulinate synthase, non-specific, mitochondrial, 5-aminolevulinate synthase, nonspecific, mitochondrial, 5-aminolevulinic acid synthase 1, aminolevulinate, delta-, synthase 1, delta-ALA synthase 1, delta-aminolevulinate synthase 1, migration-inducing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alas1 using anti-Alas1 antibody (A10405-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat NRK whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alas1 antigen affinity purified polyclonal antibody (Catalog # A10405-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alas1 at approximately 71 kDa. The expected band size for Alas1 is at 71 kDa.
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