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ProductsBack/ALCAM / CD166 Antibody (clone 5B3)

ALCAM / CD166 Antibody (clone 5B3)

LS-C169112
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetALCAM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ALCAM / CD166 Antibody (clone 5B3)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), IHC, WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B3
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID214
  • Target name
    ALCAM
  • Target description
    activated leukocyte cell adhesion molecule
  • Target synonyms
    CD166; CD166 antigen; MEMD
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD166/ALCAM using anti-CD166/ALCAM antibody (A01788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD166/ALCAM antigen affinity purified polyclonal antibody (Catalog # A01788-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD166/ALCAM at approximately 100-110 kDa. The expected band size for CD166/ALCAM is at 65 kDa.
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