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Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.

CD166 Polyclonal Antibody

BS-1251R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetALCAM
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD166 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID214
  • Target name
    ALCAM
  • Target description
    activated leukocyte cell adhesion molecule
  • Target synonyms
    CD166; CD166 antigen; MEMD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13740
  • Protein Name
    CD166 antigen
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking. Vaicek J et al., 2020 Jan 25, N Biotechnol
    Read more
  • Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells. Kovac M et al., 2017 Nov, Biotechnol Prog
    Read more

Datasheet

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Figure 1. Western blot analysis of CD166/ALCAM using anti-CD166/ALCAM antibody (A01788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD166/ALCAM antigen affinity purified polyclonal antibody (Catalog # A01788-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD166/ALCAM at approximately 100-110 kDa. The expected band size for CD166/ALCAM is at 65 kDa.
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