More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CD166 Polyclonal Antibody
Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Lane 1: human colon carcinoma lysates Lane 2: rat brain lysates probed with Anti CD166 Polyclonal Antibody, Unconjugated (bs-1251R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.

CD166 Polyclonal Antibody

BS-1251R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetALCAM
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    CD166 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID214
  • Target name
    ALCAM
  • Target description
    activated leukocyte cell adhesion molecule
  • Target synonyms
    CD166, MEMD, CD166 antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13740
  • Protein Name
    CD166 antigen
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Vašíček J, Kováč M, Baláži A, et al. Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking. N Biotechnol. 2020,54:1-12. doi: 10.1016/j.nbt.2019.08.001
    Read this paper
  • Kovac M, Vasicek J, Kulikova B, et al. Different RNA and protein expression of surface markers in rabbit amniotic fluid-derived mesenchymal stem cells. Biotechnol Prog. 2017,33(6):1601-1613. doi: 10.1002/btpr.2519
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of K562 cells using CD166 Polyclonal Antibody.
Product group Antibodies
Cusabio
ALCAM AntibodyCSB-PA005632
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetALCAM
  • SizePrice
Product group Antibodies
GeneTex
CD166 antibodyGTX130098
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetALCAM
  • SizePrice
Product group Antibodies
Biosynth
CD166 antibody10R-1985
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD166 [EJ212/007-C12-5]Ab02669-10.0
ApplicationsFunctional Assay, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetALCAM
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ALCAM Antibody144-65500
ApplicationsWestern Blot
TargetALCAM
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-ALCAMY158414
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
MedChem Express
CD166 Antibody (YA3492, Rabbit)HY-P81138
TargetALCAM
  • SizePrice
Figure 1. Western blot analysis of CD166/ALCAM using anti-CD166/ALCAM antibody (A01788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD166/ALCAM antigen affinity purified polyclonal antibody (Catalog # A01788-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD166/ALCAM at approximately 100-110 kDa. The expected band size for CD166/ALCAM is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-CD166/ALCAM Antibody Picoband(r)A01788-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetALCAM
  • SizePrice
Anti-ALCAM Antibody
Product group Antibodies
Antibodies.com
Anti-ALCAM AntibodyA97726
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Assay Genie
CD166/ALCAM AntibodyCAB2218
TargetALCAM
  • SizePrice