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Western blot All lanes: Retinal dehydrogenase 1 antibody at 4ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western blot All lanes: Retinal dehydrogenase 1 antibody at 4ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
Western blot All lanes: Retinal dehydrogenase 1 antibody at 4ug/ml + Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa

ALDH1A1 Antibody

CSB-PA001565ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetALDH1A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDH1A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID216
  • Target name
    ALDH1A1
  • Target description
    aldehyde dehydrogenase 1 family member A1
  • Target synonyms
    acetaldehyde dehydrogenase 1; ALDC; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH1; ALDH11; ALDH-E1; ALHDII; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; HEL12; HEL-9; HEL-S-53e; PUMB1; RALDH 1; RALDH1; retinal dehydrogenase 1; retinaldehyde dehydrogenase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00352
  • Protein Name
    Retinal dehydrogenase 1
  • Scientific Description
    Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Image obtained from One World Lab validations program. A549 cells were stained with ALDH1 Polyclonal Antibody, Unconjugated (bs-10162R) at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
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Figure 1. Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (A01392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified polyclonal antibody (Catalog # A01392) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A1 at approximately 55 kDa. The expected band size for ALDH1A1 is at 55 kDa.
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