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Western Blot Positive WB detected in: K562 whole cell lysate, Mouse kidney tissue All lanes: ALDH1A2 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 54, 55, 47 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, Mouse kidney tissue All lanes: ALDH1A2 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 54, 55, 47 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, Mouse kidney tissue All lanes: ALDH1A2 antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 54, 55, 47 kDa Observed band size: 54 kDa

ALDH1A2 Antibody

CSB-PA001566EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetALDH1A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDH1A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8854
  • Target name
    ALDH1A2
  • Target description
    aldehyde dehydrogenase 1 family member A2
  • Target synonyms
    DIH4, RALDH(II), RALDH2, RALDH2-T, retinal dehydrogenase 2, RALDH 2, retinaldehyde-specific dehydrogenase type 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94788
  • Protein Name
    Retinal dehydrogenase 2
  • Scientific Description
    Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently (By similarity).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDH1A2 using anti-ALDH1A2 antibody (A04961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A2 antigen affinity purified polyclonal antibody (Catalog # A04961-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A2 at approximately 57 kDa. The expected band size for ALDH1A2 is at 57 kDa.
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Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Mouse Uterus tissue lysates probed with RALDH2 Polyclonal Antibody, Unconjugated (bs-3676R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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