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Western Blot analysis of COS7 cells using ALDH1B1 Polyclonal Antibody
Western Blot analysis of COS7 cells using ALDH1B1 Polyclonal Antibody
Western Blot analysis of COS7 cells using ALDH1B1 Polyclonal Antibody

ALDH1B1 Antibody

CSB-PA000860
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey
TargetALDH1B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDH1B1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID219
  • Target name
    ALDH1B1
  • Target description
    aldehyde dehydrogenase 1 family member B1
  • Target synonyms
    ALDH5, ALDHX, aldehyde dehydrogenase X, mitochondrial, ALDH class 2, acetaldehyde dehydrogenase 5, aldehyde dehydrogenase 5, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30837
  • Protein Name
    Aldehyde dehydrogenase X, mitochondrial
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: monkey COS-7 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1B1 antigen affinity purified polyclonal antibody (Catalog # PB10037) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1B1 at approximately 55 kDa. The expected band size for ALDH1B1 is at 57 kDa.
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