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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: ALDH7A1 antibody at 4.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 56, 52 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: ALDH7A1 antibody at 4.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 56, 52 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse liver tissue, Mouse kidney tissue All lanes: ALDH7A1 antibody at 4.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 56, 52 kDa Observed band size: 59 kDa

ALDH7A1 Antibody

CSB-PA001579LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH7A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDH7A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID501
  • Target name
    ALDH7A1
  • Target description
    aldehyde dehydrogenase 7 family member A1
  • Target synonyms
    ATQ1, EPD, EPEO4, PDE, alpha-aminoadipic semialdehyde dehydrogenase, 26g turgor protein homolog, P6c dehydrogenase, alpha-AASA dehydrogenase, antiquitin-1, betaine aldehyde dehydrogenase, delta1-piperideine-6-carboxylate dehydrogenase, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49419
  • Protein Name
    Alpha-aminoadipic semialdehyde dehydrogenase
  • Scientific Description
    Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH7A1 antigen affinity purified polyclonal antibody (Catalog # PB10038) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH7A1 at approximately 55 kDa. The expected band size for ALDH7A1 is at 55 kDa.
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