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ProductsBack/Alix (G-10) Alexa Fluor(r) 546

Alix (G-10) Alexa Fluor(r) 546

SC-166952AF546
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPDCD6IP
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Alix (G-10) Alexa Fluor(r) 546
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 546
  • Gene ID10015
  • Target name
    PDCD6IP
  • Target description
    programmed cell death 6 interacting protein
  • Target synonyms
    AIP1, ALIX, DRIP4, HP95, MCPH29, programmed cell death 6-interacting protein, ALG-2 interacting protein 1, ALG-2-interacting protein X, PDCD6-interacting protein, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALIX using anti-ALIX antibody (PB9770). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified polyclonal antibody (Catalog # PB9770) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALIX at approximately 96 kDa. The expected band size for ALIX is at 96 kDa.
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