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Alkaline Phosphatase Antibody

HY-P80012
MedChem Express
TargetALPL
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Alkaline Phosphatase Antibody
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID249
  • Target name
    ALPL
  • Target description
    alkaline phosphatase, biomineralization associated
  • Target synonyms
    alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; APTNAP; AP-TNAP; HOPS; HPPA; HPPC; HPPI; HPPO; liver/bone/kidney-type alkaline phosphatase; tissue non-specific alkaline phosphatase; tissue-nonspecific ALP; TNALP; TNAP; TNSALP
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Alkaline Phosphatase Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 57 kDa, targeting to Alkaline Phosphatase. It can be used for WB,ICC,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alkaline phosphatase/ALPL using anti-Alkaline phosphatase/ALPL antibody (A01008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alkaline phosphatase/ALPL antigen affinity purified polyclonal antibody (Catalog # A01008-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alkaline phosphatase/ALPL at approximately 80KD. The expected band size for Alkaline phosphatase/ALPL is at 57KD.
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