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ALKBH1 / ALKB Antibody

LS-C401143
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetALKBH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ALKBH1 / ALKB Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8846
  • Target name
    ALKBH1
  • Target description
    alkB homolog 1, histone H2A dioxygenase
  • Target synonyms
    ABH, ABH1, ALKBH, alkB, hABH, nucleic acid dioxygenase ALKBH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase ALKBH1, DNA demethylase ALKBH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, alkB, alkylation repair homolog 1, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, alpha-ketoglutarate-dependent dioxygenase ABH1, mRNA N(3)-methylcytidine demethylase, tRNA N1-methyl adenine demethylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALKBH1 using anti-ALKBH1 antibody (A06945-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH1 antigen affinity purified polyclonal antibody (Catalog # A06945-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH1 at approximately 43KD. The expected band size for ALKBH1 is at 43KD.
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