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ALKBH1 / ALKB Antibody

LS-C815870
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetALKBH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ALKBH1 / ALKB Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (1:50 - 1:200), IP, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8846
  • Target name
    ALKBH1
  • Target description
    alkB homolog 1, histone H2A dioxygenase
  • Target synonyms
    ABH; ABH1; alkB; alkB, alkylation repair homolog 1; ALKBH; alkylated DNA repair protein alkB homolog 1; alkylation repair, alkB homolog; alpha-ketoglutarate-dependent dioxygenase ABH1; DNA 6mA demethylase; DNA demethylase ALKBH1; DNA lyase ABH1; DNA N6-methyl adenine demethylase ALKBH1; DNA oxidative demethylase ALKBH1; hABH; mRNA N(3)-methylcytidine demethylase; nucleic acid dioxygenase ALKBH1; tRNA N1-methyl adenine demethylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALKBH1 using anti-ALKBH1 antibody (A06945-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH1 antigen affinity purified polyclonal antibody (Catalog # A06945-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH1 at approximately 43KD. The expected band size for ALKBH1 is at 43KD.
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