IHC image of CSB-PA001618LA01HU diluted at 1:600 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA001618LA01HU diluted at 1:600 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ALOX12B Antibody
CSB-PA001618LA01HU
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetALOX12B
Overview
- SupplierCusabio
- Product NameALOX12B Antibody
- Delivery Days Customer20
- ApplicationsELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID242
- Target nameALOX12B
- Target descriptionarachidonate 12-lipoxygenase, 12R type
- Target synonyms12R-LOX, ARCI2, arachidonate 12-lipoxygenase, 12R-type, 12R-lipoxygenase, epidermis-type lipoxygenase 12
- HostRabbit
- IsotypeIgG
- Protein IDO75342
- Protein NameArachidonate 12-lipoxygenase, 12R-type
- Scientific DescriptionNon-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)-hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC41116161
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