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ProductsBack/alpha 1 Acid Glycoprotein antibody [AGP-47]

alpha 1 Acid Glycoprotein antibody [AGP-47]

GTX13332
GeneTex
ApplicationsDot Blot, Western Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetORM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha 1 Acid Glycoprotein antibody [AGP-47]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:10,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AGP-47
  • Conjugate
    Unconjugated
  • Gene ID5004
  • Target name
    ORM1
  • Target description
    orosomucoid 1
  • Target synonyms
    AGP 1; AGP1; AGP-A; alpha-1-acid glycoprotein 1; epididymis secretory sperm binding protein Li 153w; HEL-S-153w; OMD 1; ORM
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02763
  • Protein Name
    Alpha-1-acid glycoprotein 1
  • Scientific Description
    Alpha 1 Acid Glycoprotein is an acute-phase plasma protein that, together with haptoglobin and C-reactive protein, indicates inflammation. In human plasma, AGP (44 kDa) is found at levels of 0.5-1.4 mg/ml and pronounced changes of these levels have been observed in various pathological conditions. Increased concentrations of AGP are known to be associated with various inflammatory diseases, trauma, malignancies, myocardial infarction, rheumatoid arthritis, after major surgery, and in cases of chronic pain.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
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