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ProductsBack/AAT Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha 1 Antitrypsin Polyclonal Antibody, Unconjugated (bs-0096R) at 1:400 overnight at 4°C, DAB staining.

AAT Polyclonal Antibody

BS-0096R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSERPINA1
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Overview

  • Supplier
    Bioss
  • Product Name
    AAT Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5265
  • Target name
    SERPINA1
  • Target description
    serpin family A member 1
  • Target synonyms
    A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF, alpha-1-antitrypsin, alpha-1 antitrypsin, alpha-1 protease inhibitor, alpha-1 proteinase inhibitor, alpha-1-antiproteinase, anti-elastase, epididymis secretory sperm binding protein, protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin, serine (or cysteine) proteinase inhibitor, clade A, member 1, serpin A1, serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1, serpin peptidase inhibitor clade A member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Feng YL, Yin YX, Ding J, et al. Alpha-1-antitrypsin suppresses oxidative stress in preeclampsia by inhibiting the p38MAPK signaling pathway: An in vivo and in vitro study. PLoS One. 2017,12(3):e0173711. doi: 10.1371/journal.pone.0173711
    Read this paper

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Figure 1. Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB10097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (Catalog # PB10097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 52 kDa. The expected band size for SERPINA1 is at 47 kDa.
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