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ProductsBack/Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: 1D11C6E6]
Western blot analysis using CRYAB antibody Cat.-No AM06212SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY CRYAB cDNA (Lane 2).
Western blot analysis using CRYAB antibody Cat.-No AM06212SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY CRYAB cDNA (Lane 2).
Western blot analysis using CRYAB antibody Cat.-No AM06212SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY CRYAB cDNA (Lane 2).

Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: 1D11C6E6]

AM06212SU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCRYAB
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: 1D11C6E6]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D11C6E6
  • Gene ID1410
  • Target name
    CRYAB
  • Target description
    crystallin alpha B
  • Target synonyms
    CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2, MFM2A, MFM2B, alpha-crystallin B chain, epididymis secretory protein Li 101, heat shock protein beta-5, heat shock protein family B member 5, heat-shock 20 kD like-protein, renal carcinoma antigen NY-REN-27, rosenthal fiber component
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP02511
  • Protein Name
    Alpha-crystallin B chain
  • Scientific Description
    Alpha B Crystallin (CRYAB) mouse monoclonal antibody, clone 1D11C6E6, Ascites
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha B Crystallin/CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha B Crystallin/CRYAB2 at approximately 20KD. The expected band size for Alpha B Crystallin/CRYAB is at 20KD.
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