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Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

SCNN1A Polyclonal Antibody

BS-2957R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSCNN1A
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Overview

  • Supplier
    Bioss
  • Product Name
    SCNN1A Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6337
  • Target name
    SCNN1A
  • Target description
    sodium channel epithelial 1 subunit alpha
  • Target synonyms
    BESC2, ENaCa, ENaCalpha, LIDLS3, PHA1B1, SCNEA, SCNN1, epithelial sodium channel subunit alpha, ENaC subunit alpha, alpha ENaC-2, alpha-ENaC, alpha-NaCH, amiloride-sensitive epithelial sodium channel alpha subunit, amiloride-sensitive sodium channel subunit alpha 2, epithelial Na(+) channel subunit alpha, nasal epithelial sodium channel alpha subunit, nonvoltage-gated sodium channel 1 subunit alpha, sodium channel epithelial 1 alpha subunit, sodium channel, non voltage gated 1 alpha subunit, sodium channel, non-voltage-gated 1 alpha, sodium channel, nonvoltage-gated 1 alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP37088
  • Protein Name
    Epithelial sodium channel subunit alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chen ZZ, Gong X, Guo Q, et al. Bu Yang Huan Wu decoction prevents reperfusion injury following ischemic stroke in rats via inhibition of HIF-1 α, VEGF and promotion β-ENaC expression. J Ethnopharmacol. 2019,228:70-81. doi: 10.1016/j.jep.2018.09.017
    Read this paper
  • Scortegagna M, Berthon A, Settas N, et al. The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion. JCI Insight. 2017,2(23):pii: 97128. doi: 10.1172/jci.insight.97128.
    Read this paper

Datasheet

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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA435775(SCNN1A Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of SCNN1A using anti-SCNN1A antibody (A01413-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCNN1A antigen affinity purified polyclonal antibody (Catalog # A01413-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCNN1A at approximately 76KD. The expected band size for SCNN1A is at 76KD.
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