Alpha-Glucosidase Assay Kit (Colorimetric)

LifeSpan BioSciences

Alpha-Glucosidase Assay Kit (Colorimetric)

14

For quantitative determination of Alpha-glucosidase activity and evaluation of drug effects on its metabolism.

Spectrophotometry (visible) For quantitative determination of Alpha-glucosidase activity and evaluation of drug effects on its metabolism.

...

Alpha-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked Alpha-D-glucose residues with release of Alpha-D-glucose. Alpha-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring Alpha-glucosidase activity are becoming popular in Research and Drug Discovery. This Alpha-Glucosidase Assay Kit is designed to measure Alpha-glucosidase activity directly in biological samples without pretreatment. The improved Method utilizes p-nitrophenyl-Alpha-D-glucopyranoside that is hydrolyzed specifically by Alpha-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

-20°C

41116133