Alpha-Mannosidase (AMA) Assay Kit (Colorimetric)

LifeSpan BioSciences

Alpha-Mannosidase (AMA) Assay Kit (Colorimetric)

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For quantitative determination of Alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.

Spectrophotometry (visible) For quantitative determination of Alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.

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Alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. Alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes Alpha-mannosidosis and leads to deterioration of the central nervous system in children. This non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

2°C to 8°C

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