Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
![alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_44578_20221202_IHC-Fr_M_22121123_703.webp "alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100.")
![alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_44578_20221223_IHC-P_multiple_R_22122821_311.webp "alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_44578_20221223_IHC-P_multiple_M_22122821_625.webp "alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_44578_20221230_ICC_IF_22122901_649.webp "alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_44578_20220225_WB_w_23061202_827.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_44578_20220211_WB_M_R_brain_w_23061202_547.webp "Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:200. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636641/GTX636641_T-44515_20220120_IHC-P_M_w_23061202_865.webp "alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:200. Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
alpha Synuclein antibody [HL1242]
GTX636641
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNCA
Overview
- SupplierGeneTex
- Product Namealpha Synuclein antibody [HL1242]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1242
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID6622
- Target nameSNCA
- Target descriptionsynuclein alpha
- Target synonymsNACP, PARK1, PARK4, PD1, alpha-synuclein, I+/--synuclein, non A-beta component of AD amyloid, synuclein alpha-140, synuclein, alpha (non A4 component of amyloid precursor)
- HostRabbit
- IsotypeIgG
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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