Alpha-Synuclein, Recombinant, Human

Cosmo Bio USA

Alpha-Synuclein, Recombinant, Human

16

Research Use Only

E. coli expressed human alpha-synuclein has been widely used in structural and functional studies. Masuda et. al. (1) have reported that approximately 20% of E. coli expressed human alpha-syn is mistranslated resulting in a Cys residue instead of Tyr at position 136 and causing an increase in the levels of dimeric alpha-synuclein. CSR-SYN04 is E. coli human alpha-Synuclein (human) expressed from a plasmid in which the TAC codon coding for Tyr136 in the human protein is replaced by TAT to produce protein expressing Tyr at position 136 homogenously. It can be used as human alpha-syn under similar conditions to that of in vivo. This product can also be used for comparison (control) of alpha-Synuclein Fibrils (CSR-SYN03). Source: Human Expression System: E.coli Concentration: 1mg/mL Purity: ≥ 90% (SDS-Page) Storage: -20°C (1) Masuda M, Dohmae N, Nonaka T, Oikawa T, Hisanaga S, Goedert M, Hasegawa M. Cysteine misincorporation in bacterially expressed human alpha-synuclein. FEBS Lett. 2006 Mar 20;580(7):1775-9. doi: 10.1016/j.febslet.2006.02.032. Epub 2006 Feb 24. PMID: 16513114. Related Products

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