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alpha TTP antibody [C2C3], C-term

GTX108286
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTTPA
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha TTP antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7274
  • Target name
    TTPA
  • Target description
    alpha tocopherol transfer protein
  • Target synonyms
    alpha-tocopherol transfer protein; alphaTTP; alpha-TTP; ATTP; AVED; tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin E deficiency); TTP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49638
  • Protein Name
    Alpha-tocopherol transfer protein
  • Scientific Description
    This gene encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Self-assembled alpha-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier. Aeschimann W et al., 2017 Jul 10, Sci Rep
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  • alpha-Tocopherol transfer protein is not required for the discrimination against gamma-tocopherol in vivo but protects it from side-chain degradation in vitro. Grebenstein N et al., 2014 May, Mol Nutr Food Res
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  • alpha-Tocopherol and lipid profiles in plasma and the expression of alpha-tocopherol-related molecules in the liver of Opisthorchis viverrini-infected hamsters. Laothong U et al., 2013 Apr, Parasitol Int
    Read more

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Figure 1. Western blot analysis of TTPA/TPP1 using anti-TTPA/TPP1 antibody (A00103-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTPA/TPP1 antigen affinity purified polyclonal antibody (Catalog # A00103-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTPA/TPP1 at approximately 32,37 kDa. The expected band size for TTPA/TPP1 is at 32 kDa.
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