ALT / Alanine Transaminase Assay Kit

Arigo Biolaboratories

ALT / Alanine Transaminase Assay Kit

23

Functional Assay

2 - 100 U/l

2 U/l

Alanine Transaminase (ALT) facilitates the conversion of alanine and alpha-ketoglutarate to pyruvate and glutamate. Then pyruvate and NADH are converted to lactate and NAD by lactate dehydrogenase (LDH). Therefore, the decrease in NADH absorbance at 340 n

10 min

Research Use Only

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

-20°C

41116133