AMCA-6-dUTP
ORB178359
Estimated Purity≥95%
Product group Chemicals
Molecular WeightTheoretical MW: 851.58 g/mol (free acid); Detected MW: 851.16 g/mol (free acid)
Overview
- SupplierBiorbyt
- Product NameAMCA-6-dUTP
- Delivery Days Customer10
- Application Supplier NoteIncorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 350 nm, lambdaem 450 nm, epsilon 19.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
- CertificationResearch Use Only
- Concentration1.0-1.1 mM
- Estimated Purity≥95%
- Molecular WeightTheoretical MW: 851.58 g/mol (free acid); Detected MW: 851.16 g/mol (free acid)
- Scientific DescriptionAMCA-6-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended AMCA-6-dUTP/dTTP ratio for PCR and Nick Translation: 20-30% AMCA-6-dUTP/80-70% dTTP (PCR), 30-50% AMCA-6-dUTP/70-50% dTTP (Nick Translation).
- Storage Instruction-20°C
- UNSPSC12352200