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AMHR2 Rabbit mAb

A8813
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAMHR2
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Overview

  • Supplier
    ABclonal
  • Product Name
    AMHR2 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID269
  • Target name
    AMHR2
  • Target description
    anti-Mullerian hormone receptor type 2
  • Target synonyms
    AMHR, MISR2, MISRII, MRII, anti-Muellerian hormone type-2 receptor, AMH type II receptor, MIS type II receptor, Muellerian inhibiting substance type II receptor, Mullerian inhibiting substance type II receptor, anti-Muellerian hormone type II receptor, anti-Mullerian hormone receptor, type II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16671
  • Protein Name
    Anti-Muellerian hormone type-2 receptor
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMHR2 using anti-AMHR2 antibody (PB9984). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMHR2 antigen affinity purified polyclonal antibody (Catalog # PB9984) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMHR2 at approximately 65 kDa. The expected band size for AMHR2 is at 65 kDa.
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