Aminoallyl-dUTP - Solid [116840-18-7]

Biorbyt

Aminoallyl-dUTP - Solution [116840-18-7]

16

Incorporation into DNA/cDNA by: PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: lambdamax 289 nm, epsilon 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Research Use Only

Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.

-20°C

12352200