Aminoallyl-dUTP-XX-Cy3

Biorbyt

Aminoallyl-dUTP-XX-Cy3

16

Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 550 nm, lambdaem 570 nm, epsilon 150.0 L mmol-1, cm-1 (Tris-HCl pH 7.5), CF 2600.08.

Research Use Only

1.0-1.1 mM

Liquid

Aminoallyl-dUTP-XX-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy3. Recommended Aminoallyl-dUTP-XX-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-Cy3/50% dTTP.

-20°C

12352200