Aminoallyl-dUTP-XX-Cy5

Jena Bioscience

Aminoallyl-dUTP-XX-Cy5

5

Research Use Only

Aminoallyl-dUTP-XX-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-Cy5/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-Cy5/ 80-70% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

-20°C

41116133