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ProductsBack/Amisyn / STXBP6 Antibody (aa2-210)

Amisyn / STXBP6 Antibody (aa2-210)

LS-C371880
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSTXBP6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Amisyn / STXBP6 Antibody (aa2-210)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29091
  • Target name
    STXBP6
  • Target description
    syntaxin binding protein 6
  • Target synonyms
    HSPC156, amisyn, syntaxin-binding protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STXBP6 using anti-STXBP6 antibody (A11292-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP6 antigen affinity purified polyclonal antibody (Catalog # A11292-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STXBP6 at approximately 26 kDa. The expected band size for STXBP6 is at 24 kDa.
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