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Western blot All lanes: Angiomotin antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 119, 73 kDa Observed band size: 119 kDa
Western blot All lanes: Angiomotin antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 119, 73 kDa Observed band size: 119 kDa
Western blot All lanes: Angiomotin antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 119, 73 kDa Observed band size: 119 kDa

AMOT Antibody

CSB-PA001677ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAMOT
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Overview

  • Supplier
    Cusabio
  • Product Name
    AMOT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID154796
  • Target name
    AMOT
  • Target description
    angiomotin
  • Target synonyms
    angiomotin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4VCS5
  • Protein Name
    Angiomotin
  • Scientific Description
    Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Angiomotin/AMOT using anti-Angiomotin/AMOT antibody (A04170-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiomotin/AMOT antigen affinity purified polyclonal antibody (Catalog # A04170-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiomotin/AMOT at approximately 140 kDa. The expected band size for Angiomotin/AMOT is at 118 kDa.
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