More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/AMPK alpha-1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-10344R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

AMPK alpha-1 Polyclonal Antibody

BS-10344R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRKAA1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    AMPK alpha-1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5562
  • Target name
    PRKAA1
  • Target description
    protein kinase AMP-activated catalytic subunit alpha 1
  • Target synonyms
    AMPK, AMPK alpha 1, AMPKa1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK subunit alpha-1, AMPK, alpha, 1, HMGCR kinase, acetyl-CoA carboxylase kinase, hydroxymethylglutaryl-CoA reductase kinase, protein kinase, AMP-activated, alpha 1 catalytic subunit, tau-protein kinase PRKAA1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13131
  • Protein Name
    5'-AMP-activated protein kinase catalytic subunit alpha-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Pan Y et al., 2020 Jul 15, Int J Biol Macromol
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA071831(PRKAA1 Antibody) at dilution 1/80, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
PRKAA1 AntibodyCSB-PA071831
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKAA1
  • SizePrice
Product group Antibodies
GeneTex
AMPK alpha 1 antibodyGTX112999
ApplicationsWestern Blot
TargetPRKAA1
  • SizePrice
Product group Antibodies
MedChem Express
AMPK alpha 1 Antibody (YA624, Rabbit)HY-P80376
TargetPRKAA1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-AMPKa1/2-PT Antibody130-10453
ApplicationsELISA
TargetPRKAA1
  • SizePrice
Product group Antibodies
Biosynth
AMPK alpha 1 antibody70R-51612
  • SizePrice
Figure 1. Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 64 kDa. The expected band size for AMPK alpha 1 is at 64 kDa.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetPRKAA1
  • SizePrice
Anti-AMPK alpha 1 Antibody
Product group Antibodies
Antibodies.com
Anti-AMPK alpha 1 AntibodyA13395
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-AMPK alpha 1 Antibody, Affinity PurifiedA300-507A
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetPRKAA1
  • SizePrice
Product group Antibodies
Assay Genie
MERTK Monoclonal AntibodyCAB12294
TargetPRKAA1
  • SizePrice
Product group Antibodies
Biomatik
PRKAA1 Polyclonal AntibodyCAC14615
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetPRKAA1
  • SizePrice