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ProductsBack/Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI4C3]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human APP plasmid (RC221339, hAPP), mouse APP plasmid (MR210090, mAPP), rat APP plasmid (RR204173, rAPP) using anti-APP antibody TA501006 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human APP plasmid (RC221339, hAPP), mouse APP plasmid (MR210090, mAPP), rat APP plasmid (RR204173, rAPP) using anti-APP antibody TA501006 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human APP plasmid (RC221339, hAPP), mouse APP plasmid (MR210090, mAPP), rat APP plasmid (RR204173, rAPP) using anti-APP antibody TA501006 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI4C3]

CF501006
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPP
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI4C3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4C3
  • Gene ID351
  • Target name
    APP
  • Target description
    amyloid beta precursor protein
  • Target synonyms
    AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, amyloid-beta precursor protein, alzheimer disease amyloid A4 protein homolog, alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, amyloid beta A4 protein, amyloid precursor protein, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, cerebral vascular amyloid peptide, peptidase nexin-II, protease nexin-II, testicular tissue protein Li 2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP05067
  • Protein Name
    Amyloid-beta precursor protein
  • Scientific Description
    Carrier-free (BSA/glycerol-free) APP mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of APP/C99 using anti-APP/C99 antibody (A00081-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP/C99 antigen affinity purified polyclonal antibody (Catalog # A00081-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP/C99 at approximately 99KD. The expected band size for APP/C99 is at 87KD.
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