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ProductsBack/Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI5C5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP. Positive lysates LY424694 (100ug) and LC424694 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP. Positive lysates LY424694 (100ug) and LC424694 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APP (RC221339, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APP. Positive lysates LY424694 (100ug) and LC424694 (20ug) can be purchased separately from OriGene.

Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI5C5]

CF500972
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPP
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: OTI5C5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5C5
  • Gene ID351
  • Target name
    APP
  • Target description
    amyloid beta precursor protein
  • Target synonyms
    AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, amyloid-beta precursor protein, alzheimer disease amyloid A4 protein homolog, alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, amyloid beta A4 protein, amyloid precursor protein, beta-amyloid peptide, beta-amyloid peptide(1-40), beta-amyloid peptide(1-42), beta-amyloid precursor protein, cerebral vascular amyloid peptide, peptidase nexin-II, protease nexin-II, testicular tissue protein Li 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05067
  • Protein Name
    Amyloid-beta precursor protein
  • Scientific Description
    Carrier-free (BSA/glycerol-free) APP mouse monoclonal antibody, clone OTI5C5 (formerly 5C5)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of APP/C99 using anti-APP/C99 antibody (A00081-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP/C99 antigen affinity purified polyclonal antibody (Catalog # A00081-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP/C99 at approximately 99KD. The expected band size for APP/C99 is at 87KD.
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