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Western Blot analysis of COLO205 cells using APC5 Polyclonal Antibody
Western Blot analysis of COLO205 cells using APC5 Polyclonal Antibody
Western Blot analysis of COLO205 cells using APC5 Polyclonal Antibody

ANAPC5 Antibody

CSB-PA000899
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetANAPC5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANAPC5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51433
  • Target name
    ANAPC5
  • Target description
    anaphase promoting complex subunit 5
  • Target synonyms
    APC5, anaphase-promoting complex subunit 5, cyclosome subunit 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJX4
  • Protein Name
    Anaphase-promoting complex subunit 5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Apc5/ANAPC5 using anti-Apc5/ANAPC5 antibody (A08280-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apc5/ANAPC5 antigen affinity purified polyclonal antibody (Catalog # A08280-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apc5/ANAPC5 at approximately 85KD. The expected band size for Apc5/ANAPC5 is at 85KD.
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