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ProductsBack/ANGPTL3 Recombinant Antibody, AbBy Fluor-647 Conjugated

ANGPTL3 Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62299R-BF647
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetANGPTL3
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Overview

  • Supplier
    Bioss
  • Product Name
    ANGPTL3 Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID27329
  • Target name
    ANGPTL3
  • Target description
    angiopoietin like 3
  • Target synonyms
    ANG-5, ANGPT5, ANL3, FHBL2, angiopoietin-related protein 3, angiopoietin 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5C1
  • Protein Name
    Angiopoietin-related protein 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANGPTL3 using anti-ANGPTL3 antibody (A02929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL3 antigen affinity purified polyclonal antibody (Catalog # A02929) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPTL3 at approximately 54KD. The expected band size for ANGPTL3 is at 54KD.
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