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Western Blot analysis of COLO205 cells using CARP Polyclonal Antibody
Western Blot analysis of COLO205 cells using CARP Polyclonal Antibody
Western Blot analysis of COLO205 cells using CARP Polyclonal Antibody

ANKRD1 Antibody

CSB-PA001217
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetANKRD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANKRD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27063
  • Target name
    ANKRD1
  • Target description
    ankyrin repeat domain 1
  • Target synonyms
    ALRP, C-193, CARP, CVARP, MCARP, bA320F15.2, ankyrin repeat domain-containing protein 1, ankyrin repeat domain 1 (cardiac muscle), cardiac ankyrin repeat protein, cytokine-inducible gene C-193 protein, cytokine-inducible nuclear protein, epididymis secretory sperm binding protein, liver ankyrin repeat domain 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15327
  • Protein Name
    Ankyrin repeat domain-containing protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ANKRD1 using anti-ANKRD1 antibody (A04671-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANKRD1 antigen affinity purified polyclonal antibody (Catalog # A04671-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANKRD1 at approximately 36 kDa. The expected band size for ANKRD1 is at 36 kDa.
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