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ProductsBack/Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8]
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA1-Knockout hela cells (KO, Cat# LC810011) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody TA500967. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA1-Knockout hela cells (KO, Cat# LC810011) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody TA500967. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA1-Knockout hela cells (KO, Cat# LC810011) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody TA500967. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8]

CF500967
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetANXA1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Annexin A1 (ANXA1) Mouse Monoclonal Antibody [Clone ID: OTI3A8]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3A8
  • Gene ID301
  • Target name
    ANXA1
  • Target description
    annexin A1
  • Target synonyms
    ANX1, LPC1, annexin A1, annexin I (lipocortin I), annexin-1, calpactin II, calpactin-2, chromobindin-9, epididymis secretory sperm binding protein, phospholipase A2 inhibitory protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04083
  • Protein Name
    Annexin A1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ANXA1 mouse monoclonal antibody, clone OTI3A8 (formerly 3A8)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (A01451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (Catalog # A01451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 35 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.
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