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Annexin A3 (ANXA3) Polyclonal Antibody

CAU22674
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetAnxa3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Annexin A3 (ANXA3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25291
  • Target name
    Anxa3
  • Target description
    annexin A3
  • Target synonyms
    Anx3, LC3, LRRGT00047, annexin A3, 35-alpha calcimedin, Annexin III (Lipocortin III), PAP-III, annexin-3, lipocortin III, placental anticoagulant protein III
  • Host
    Rabbit
  • Protein IDP14669
  • Protein Name
    Annexin A3
  • Scientific Description
    The Annexin A3 (ANXA3) Polyclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Rat
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANXA3 using anti-ANXA3 antibody (PA1510). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates. Lane 2: human Hela whole cell lysates. Lane 3: human A431 whole cell lysates. Lane 4: human SH-SY5Y whole cell lysates. Lane 5: rat lung tissue lysates. Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA3 antigen affinity purified polyclonal antibody (Catalog # PA1510) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA3 at approximately 36 kDa. The expected band size for ANXA3 is at 36 kDa.
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